M7154
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M7154
Sigma
2-Mercaptoethanol
for electrophoresis
| Synonym: | β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol |
| CAS Number: | 60-24-2 |
| Linear Formula: | HSCH2CH2OH |
| Molecular Weight: | 78.13 |
| Beilstein Registry Number: | 773648 |
| EC Number: | 200-464-6 |
| MDL number: | MFCD00004890 |
| PubChem Substance ID: | 24897143 |
Description
| Application | BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. |
Properties
| grade | for electrophoresis |
| vapor density | 2.69 (vs air) |
| vapor pressure | 1 mmHg ( 20 °C) |
| expl. lim. | 18 % |
| concentration | 14.3 M (pure liquid) |
| refractive index | n |
| bp | 157 °C(lit.) |
| density | 1.114 g/mL at 25 °C(lit.) |
| cation traces | Cu: <1 ppm |
| Fe: <1 ppm | |
| Pb: <5 ppm | |
| Zn: <1 ppm |
Safety
| Personal Protective Equipment | Faceshields, Gloves, Goggles, Respirators |
| Hazard Codes | T,N |
| Risk Statements | 20/22-24-34-51/53 |
| Safety Statements | 26-36/37/39-45-61 |
| RIDADR | UN2966 6.1/PG 2 |
| WGK Germany | 3 |
| RTECS | KL5600000 |
| UEL | 18.00%(V) |
| LEL | 2.30%(V) |
| Flash Point(F) | 170.6 °F |
| Flash Point(C) | 77 °C |
References
| Reference | Hames, B.D. and Rickwood, D. Gel Electrophoresis of Proteins: A Practical Approach 2nd ed., New York, NY , (1990), 32-35 |
| Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685, (1970) Abstract | |
| Merck | Merck 13,5894 |
| Beilstein | Beil. 1,IV,2428 |
| reference | Aldrich MSDS 1, 1162:C / Corp MSDS 1 (2), 2209:A / FT-IR 2 (1), 415:B / FT-IR 1 (1), 262:B / FT-NMR 1 (1), 422:B / IR-Spectra (2), 138:E / IR-Spectra (3), 154:F / NMR-Reference 2 (1), 228:B / RegBook 1 (1), 281:L / Sax 6, 1744 / Sigma FT-IR 1 (2), 700:C / Structure Index 1, 38:D:7 / Vapor Phase 3, 344:C |
